Discovery and Engineering of an Aldehyde Tolerant 2-deoxy-D-ribose 5-phosphate Aldolase (DERA) from Pectobacterium atrosepticum
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چکیده
منابع مشابه
Preparative scale enzymatic synthesis of D-sedoheptulose-7-phosphate from -hydroxypyruvate and D-ribose-5-phosphate
An enzymatic method for ready access to D-sedoheptulose-7-phosphate on a preparative scale was developed, based on the irreversible transketolase-catalyzed reaction: β-hydroxypyruvate + D-ribose-5phosphate → D-sedoheptulose-7-phosphate. D-Sedoheptulose-7-phosphate disodium salt was obtained in 81% overall yield determined using a standard curve obtained by LC/MS/MS.
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DERA, 2-deoxyribose-5-phosphate aldolase, catalyzes the retro-aldol cleavage of 2-deoxy-ribose-5phosphate (dR5P) into glyceraldehyde-3-phosphate (G3P) and acetaldehyde in a branch of the pentose phosphate pathway. In addition to the physiological reaction, DERA also catalyzes the reverse addition reaction and, hence, is an interesting candidate for bio-catalysis of carbo-ligation reactions, whi...
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The 2-deoxy-d-ribose-5-phosphate aldolase (DERA) offers access to highly desirable building blocks for organic synthesis by catalyzing a stereoselective C-C bond formation between acetaldehyde and certain electrophilic aldehydes. DERA´s potential is particularly highlighted by the ability to catalyze sequential, highly enantioselective aldol reactions. However, its synthetic use is limited by t...
متن کاملThymidine phosphorylase, 2-deoxy-D-ribose and angiogenesis.
Angiogenesis is the term used to describe the formation of new blood vessels from the existing vasculature. In order to attract new vessels, a tissue must release an endothelial-cell chemoattractant. 2-Deoxy-D-ribose is produced in vivo by the catalytic action of thymidine phosphorylase (TP) on thymidine and has recently been identified as an endothelial-cell chemoattractant and angiogenesis-in...
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We report the annotated genome sequence of the enterobacterial plant pathogen Pectobacterium atrosepticum strain 21A, isolated in Belarus from potato stem with blackleg symptoms.
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ژورنال
عنوان ژورنال: Catalysts
سال: 2020
ISSN: 2073-4344
DOI: 10.3390/catal10080883